ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR

ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR

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Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms.They are still difficult to cultivate on laboratory media, which highlights the Vinegar importance of alternative methods of detection of these bacteria.The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in NEFF N50 N1AHA01N0B Warming Drawer - Stainless Steel wine.

We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit.The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.